Assembly of polymorphic genomes: Algorithms and application to Ciona savignyi
نویسندگان
چکیده
منابع مشابه
Assembly of polymorphic genomes: algorithms and application to Ciona savignyi.
Whole-genome assembly is now used routinely to obtain high-quality draft sequence for the genomes of species with low levels of polymorphism. However, genome assembly remains extremely challenging for highly polymorphic species. The difficulty arises because two divergent haplotypes are sequenced together, making it difficult to distinguish alleles at the same locus from paralogs at different l...
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Diploid genome reconstruction of Ciona intestinalis and comparative analysis with Ciona savignyi.
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We present Meraculous2, an update to the Meraculous short-read assembler that includes (1) handling of allelic variation using " bubble " structures within the de Bruijn graph, (2) improved gap closing, and (3) an improved scaffolding algorithm that produces more complete assemblies without compromising scaffolding accuracy. The speed and bandwidth efficiency of the new parallel implementation ...
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ژورنال
عنوان ژورنال: Genome Research
سال: 2005
ISSN: 1088-9051
DOI: 10.1101/gr.3722605